Table 2. Major differences in substrates giving dye reduction between spoligotypes of M .bovis and within spoligotypes of M .bovis.

		Between M. bovis spoligotypes						Within spoligotypes
PM1 plate		9 vs 17		17 vs 35		9 vs 35
A03	N-ac-D-Glucosamine		9b>17c				9b>35a	9b>9c
B03	Glycerol	9>17	9b>17c	35a>17				9b>9a = 9c
C09	D-Glucose						9b>35a
E05	Tween 80							9b>9a
G09	monomethylsuccinate			17>35a	17c>35a	9>35a	9b>35a
G10	methylpyruvate					9>35a	9b>35a
PM2A plate
D06	D-Tagatose							9b>9a
E02	Caproic acid			35a>17	35a>17c
PM3B plate
B10	L-Serine							17c>17b
E08	D-Glucosamine							17c>17b

All differences were significant at P<0.05. There was at least a 5-fold difference in dye reduction (Omnilog units) between the strains. Dye reduction was >10 Omnilog units for the strain with the highest value. Differences were tested between spoligotypes by (i) comparing pooled dye reduction data for all strains tested and (ii) comparing data from the most used strains: 9b, 17c and 35a. Note that 35a was the only representative of spoligotype 35. Given that there are ten pairwise differences, these data are shown in a table rather than heatmaps.