Table 3. Growth of M. tuberculosis and M. bovis in glucose and Tween 80 as sole usable carbon sources in liquid media.

Medium	Sauton		Roisin's
Bacteria	M. bovis	M. tuberculosis	M. bovis	M. tuberculosis
Glucose (2 g/l)+tyloxapol (0.25 ml/l)	<1	450–600	<1	78–96
Glucose (2 g/l)+Tween 80 (0.1 ml/l)	90–126	450–720	60–82	96–102
Tween 80 (0.1 ml/l)	21–30	24–33	17–19	18–21

Bottles with 10 ml culture medium were set up to investigate synergistic use of hexoses and Tween 80 suggested by Biolog PM data. Tween 80 is a source of fatty acids and a detergent while tyloxapol only acts as a detergent. Strains used in Sauton medium: M. bovis: AF2122/97 (9b), 61/0038/01 (9a), 21/7917/05 (17c) and 61/1307/01 (35a); M. tuberculosis H37Rv and Tb30. Only AF2122/97 and H37Rv were used in Roisin's medium. Values are growth yield (mg dry weight bacteria/l culture) after 25 day culture. A range is given for all strains- duplicate cultures were done.