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. 2013 Jan 10;8(1):e50504. doi: 10.1371/journal.pone.0050504

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© 2013 Mithu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Magnetisation build-up curves of (a) C -C , (b) C -C , (c) C -C , (d) C -C , (e) C -CO, (f) C -CO, (g) C -CO, and (h) C -C cross-peaks of U-¹³C-,¹⁵N-L-histidineHO for different recoupling schemes applied on the ¹H channel, at = 30 kHz and = 16.43 T. Each recoupling scheme was applied for a fixed mixing-time period (90 ms) using different RF amplitudes of 7.3, 16, 24, 30, and 40 kHz. values calculated for individual cross-peaks according to Eq. 1 are plotted as a function of RF amplitude. values are normalised with respect to the maximum value yielded by PARIS (N = 1/2) ( = 16 kHz) in (a, b, and c), PARIS-xy (m = 1)(N = 2) ( = 30 kHz) in (d), PARIS (N = 2) ( = 30 kHz) in (e and f), PARIS-xy (m = 2)(N = 1/2) ( = 30 kHz) in (g), and PARIS (N = 1/2) ( = 40 kHz) in (h). Data points are joined with straight lines to guide the readers' eyes.

Inline graphic — Magnetisation build-up curves of (a) C -C , (b) C -C , (c) C -C , (d) C -C , (e) C -CO, (f) C -CO, (g) C -CO, and (h) C -C cross-peaks of U-¹³C-,¹⁵N-L-histidineHO for different recoupling schemes applied on the ¹H channel, at = 30 kHz and = 16.43 T. Each recoupling scheme was applied for a fixed mixing-time period (90 ms) using different RF amplitudes of 7.3, 16, 24, 30, and 40 kHz. values calculated for individual cross-peaks according to Eq. 1 are plotted as a function of RF amplitude. values are normalised with respect to the maximum value yielded by PARIS (N = 1/2) ( = 16 kHz) in (a, b, and c), PARIS-xy (m = 1)(N = 2) ( = 30 kHz) in (d), PARIS (N = 2) ( = 30 kHz) in (e and f), PARIS-xy (m = 2)(N = 1/2) ( = 30 kHz) in (g), and PARIS (N = 1/2) ( = 40 kHz) in (h). Data points are joined with straight lines to guide the readers' eyes.