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. 2013 Jan 10;8(1):e53750. doi: 10.1371/journal.pone.0053750

Figure 1. PRMT6 protein-protein interaction domain maps mainly to the N-terminal protein portion 1–86.

Figure 1

(A) Schematic representation of the PRMT6 deletion mutants. The central region containing the catalytic domain is indicated in black. Numbers correspond to the aminoacids positions. Positive interactions out of 31 partners tested are indicated on the right. (B) Total protein extracts from yeast expressing the different PRMT6 deletion mutants in fusion with LexA DNA binding domain, grown in medium containing glucose (Glu) or galactose and raffinose (Gal/Raf), were separated in SDS-PAGE (T = 10%) and analyzed by western blot using an α-LexA antibody. Molecular weights are indicated on the left.