Figure 4. Discovering new substrates for PRMT6.

(A) Recombinant PRMT6 partners in fusion with GST were incubated with radio-labelled S-adenosyl-L-(methyl-³H)-methionine and GST-PRMT6 for in vitro methylation assay (lanes 2–6, 8, 9). GST and GST-GAR (lanes 1 and 7) were used as negative and positive control, respectively. Proteins were separated by SDS-PAGE (T = 10%) and checked by fluorography. Experiments were repeated at least twice and a representative result is shown. (B) Blue Comassie staining was used to check both the correct production and the amount of recombinant proteins. Arrows indicate the position of PRMT6.