Table 1.
Kinetic constants of LC/B and LC/T on VAMP2 point mutations
| VAMP P sites | Wt | P7, L70 | P6, Q71 | P1,Q76 | P1′, F77 | P2′, E78 | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Point Mutation | No | A | A | A | Y | F | R | E | A | A | D | R | Y |
| Km (μM) | 1.7± 0.3 | 6.5± 0.8 | 1.9± 0.2 | 1.8± 0.3 | 1.7± 0.3 | 1.8± 0.4 | 1.7± 0.4 | 1.8± 0.4 | 1.6± 0.2 | 2.4± 0.4 | 2.6± 0.2 | 2.2± 0.2 | 1.8± 0.2 |
| LC/B | |||||||||||||
| kcat (min−1) | 66.0 | 1.2 | 4.4 | 9.6 | 33.2 | 11.0 | 5.6 | 1.3 | 0.2 | 12.2 | 67.3 | 1.2 | 1.3 |
| kcat/Km (min−1 • μM−1) | 39.0 | 0.2 | 2.3 | 5.3 | 19.5 | 6.1 | 3.3 | 0.7 | 0.1 | 5.1 | 25.9 | 0.6 | 0.7 |
| Km (μM) | 4.1±0.2 | 20.8±0.8 | 4.1±1.2 | 4.0±0.6 | 4.1±0.2 | 4.1±0.3 | 4.0±0.2 | 4.1±0.6 | 3.8±0.4 | 4.3±0.4 | 4.1±0.4 | 3.9±0.3 | N/D |
| LC/T | |||||||||||||
| kcat (min−1) | 9.6 | 0.1 | 1.6 | 1.3 | 9.4 | 9.4 | 1.5 | 1.9 | 0.02 | 0.05 | 9.7 | 0.2 | N/D |
| kcat/Km (min−1 • μM−1) | 2.3 | 0.01 | 0.4 | 0.3 | 2.3 | 2.3 | 0.4 | 0.5 | 0.005 | 0.01 | 2.4 | 0.05 | N/D |