. Author manuscript; available in PMC: 2013 May 8.

Published in final edited form as: Biochemistry. 2012 Apr 24;51(18):3941–3947. doi: 10.1021/bi3000098

Table 2.

Kinetic constants of LC/B, LC/T and LC derivatives

LCs	VAMP2 (Residue)	LC Pockets	LC derivative	K_m (μM)	k_cat (min⁻¹)	k_cat/K_m (min⁻¹ • μM⁻¹)
LC/B			Wt-LC/B	1.7±0.3	66	39

	P7(L⁷⁰)	S7	F²⁶A	9.1±0.8	5.6	0.6
			F²⁶D	35.6±2.4	0.4	0.01
			F²⁶Y	1.8±0.3	68	39

	P6 (Q⁷¹)	S6	N¹⁷⁹L	1.7±0.4	16	9.4

	P1 (Q⁷⁶)	S1	E¹⁶⁸A	1.7±0.4	3.2	1.9
			E¹⁶⁸K	1.6±0.2	0.4	0.3
			N¹⁶⁹A	1.8±0.3	6.5	3.6
			E¹⁷⁰A	1.8±0.4	6.6	3.7

	P1′(F⁷⁷)	S1′	I²²⁷A	1.6±0.2	0.8	0.5
			I²²⁷E	1.8±0.3	0.08	0.05
			I²²⁷L	1.8±0.4	0.1	0.07

	P2′ (E⁷⁸)	S2′	R³⁷⁰A	1.8±0.4	0.6	0.4
	P2′ (E⁷⁸)	S2′	R³⁷⁰E	1.8±0.2	0.2	0.08

LC/T			Wt-LC/T	4.1±0.2	9.6	2.3

	P7(L⁷⁰)	S7	Y²⁶A	20.8±0.8	0.9	0.05
			Y²⁶D	N/D	N/D	N/D
			Y²⁶F	4.2±0.2	9.9	2.4

	P6 (Q⁷¹)	S6	N¹⁸³L	4.2±0.4	4.5	1.1

	P1 (Q⁷⁶)	S1	K¹⁶⁸A	4.2±0.2	10.6	2.5
			K¹⁶⁸E	4.1±0.2	79.8	19.5
			N¹⁶⁹A	4.0±0.2	2.0	0.5
			E¹⁷⁰A	4.1±0.6	2.6	0.63

	P1′(F⁷⁷)	S1′	L²³¹A	4.2±0.2	0.3	0.8
			L²³¹K	N/D^*	N/D	N/D
			P²⁰⁵A	4.0±0.3	0.3	0.8
			P²⁰⁵K	N/D	N/D	N/D

	P2′ (E⁷⁸)	S2′	R³⁷⁴A	N/D	N/D	N/D
	P2′ (E⁷⁸)	S2′	R³⁷⁴E	N/D	N/D	N/D

N/D, Not determined, the mutant is too inactive to determine the kinetic constants