. Author manuscript; available in PMC: 2013 May 8.

Published in final edited form as: Biochemistry. 2012 Apr 24;51(18):3941–3947. doi: 10.1021/bi3000098

Table 3.

Compensatory mutational assay for LC/B and LC/T interactions with VAMP2

LCs	50% Cleavage of VAMP2 (nM)	Ratio of activity (LC/LC derivative)	50% Cleavage of VAMP2 Q⁷¹A (nM)	Ratio of activity (LC/LC derivative)
LC/B	6	4	96	1/16
LC/B(N¹⁷⁹L)	24	4	6	1/16
LC/T	120	2	720	1/6
LC/T(N¹⁸³L)	240	2	120	1/6

			50% Cleavage of VAMP2 G⁷³A (nM)

LC/B	6	1	192	1/32
LC/B(R¹⁸⁴M)	6	1	6	1/32
LC/T	120	1	7680	1/64
LC/T(R¹⁸⁸M)	20	1	60	1/64

			50% Cleavage of VAMP2 E⁷⁸R (nM)

LC/B	6	400	300	1/50
LC/B(R³⁷⁰E)	2400	400	6	1/50
LC/T	120	300	>36000	ND*
LC/T(R³⁷⁴E)	36000	300	>36000	ND*

ND*, Not determined