Table. 10-plex synthetic biomarker library.
List of synthetic biomarkers (G1–G10), protease substrates (S1–S10) and isobaric mass reporters (R1–R10) used in study.
| Synthetic Biomarker Library[a],[b] (G1–G10) | Substrate (S1–S10) | Isobaric mass code[c],[d] (R1–R10) | y6 reporter | [y6+H+] |
|---|---|---|---|---|
| e+3G+6VndneeGFfsAr-X-K(FAM)GGPQGIWGQC-NW | PQGIWGQ | e+3G+6VndneeGFfsAr | GFfsAr | 683.4 |
| e+2G+6Vndnee+1GFfsAr-X-K(FAM)GGLVPRGSGC-NW | LVPRGSG | e+2G+6Vndnee+1GFfsAr | +1GFfsAr | 684.4 |
| e+1G+6Vndnee+2GFfsAr-X-K(FAM)GGPVGLIGC-NW | PVGLIG | e+1G+6Vndnee+2GFfsAr | +2GFfsAr | 685.4 |
| eG+6Vndnee+2GFfs+1Ar-X-K(FAM)GGPWGIWGQGC-NW | PWGIWGQG | eG+6Vndnee+2GFfs+1Ar | +2GFfs+1Ar | 686.4 |
| eG+5VndneeGFfs+4Ar-X-K(FAM)GGPVPLSLVMC-NW | PVPLSLVM | eG+5VndneeGFfs+4Ar | GFfs+4Ar | 687.4 |
| e+3G+1Vndnee+1GFfs+4Ar-X-K(FAM)GGPLGLRSWC-NW | PLGLRSW | e+3G+1Vndnee+1GFfs+4Ar | +1GFfs+4Ar | 688.4 |
| e+3GVndneeG+6FfsAr-X-K(FAM)GGPLGVRGKC-NW | PLGVRGK | e+3GVndneeG+6FfsAr | G+6FfsAr | 689.4 |
| e+2GVndneeG+6Ffs+1Ar-X-K(FAM)GGf(Pip)RSGGGC-NW | f(Pip)RSGGG | e+2GVndneeG+6Ffs+1Ar | G+6Ffs+1Ar | 690.4 |
| e+1GVndnee+2G+6FfsAr-X-K(FAM)GGfPRSGGGC-NW | fPRSGGG | e+1GVndnee+2G+6FfsAr | +2G+6FfsAr | 691.4 |
| eGVndnee+3G+6FfsAr-X-K(FAM)GGf(Pip)KSGGGC-NW | f(Pip)KSGGG | eGVndnee+3G+6FfsAr | +3G+6FfsAr | 692.4 |
[a]
X (3-amino-3-(2-nitrophenyl)propionic acid), FAM (carboxyfluorescein), Pip (pipecolic acid), NW (nanoworm)
[b]
lower case = d-amino acid
[c]
photocleaved C-terminus = CONH2
[d]
mass= 1589.8 Da