Figure 3.

Properties of p-LTD

(A) Input-specific p-LTD with BAPTA (30 mM) in the postsynaptic recording pipette. Insets show EPSP before (1) and after (2) p-LTD (left) and the membrane potential response during the protocol (right). (B) Summary of results. (C) p-LTD requires NMDA receptors. p-LTD is completely blocked in 50 μM d-AP5 (gray squares). (D) Summary of results. (E) p-LTD does not require activation of mGlu or CB1 receptors. p-LTD induced by a 3S + S protocol in control slices (black triangles) and in slices treated with the mGluR antagonist LY341495 (100 μM; gray triangles) or the CB1 receptor antagonist AM251 (3 μM; open triangles) is shown. Insets show EPSP before (1 and 1′) and 30 min after (2 and 2′) p-LTD induction protocol in LY341495-treated slices (1 and 2) and in AM251-treated slices (1′ and 2′). (F) Summary of results. The number of slices used for each condition is indicated in parentheses at the top of each bar. Error bars represent SEM. ^∗∗p < 0.01, Student’s t test. See also Figures S2 and S3.