Fig. 3.
Effect of resveratrol on triglyceride and GPDH activity in 3T3-L1 cells. 3T3-L1 cells were plated at a density of 2.0 × 104 cells/mLin a 6 well plate with DMEM supplemented with 10% BCS for 2 days, the monolayers were applied to differentiation induction with DMEM supplemented with 10% FBS, 10 µg/mLinsulin, 1 µmol/L Dex, 0.5 mmol/L IBMX for 2 days. After differentiation induction, the monolayer was incubated in post differentiation medium with 0, 10, 20, and 40 µmol/L resveratrol. Triglyceride accumulation was estimated by the commercial triglyceride assay kit (A). GPDH activity was estimated by a commercial GPDH activity assay kit (B). Each bar represents the mean ± SE. Comparison among different concentrations of resveratrol that yielded significant differences (P < 0.05) are indicated by different letters above each bar.