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. 2013 Jan 10;92(1):28–40. doi: 10.1016/j.ajhg.2012.11.013

Figure 5.

Figure 5

Flow Cytometry Analysis of FcγRIIb Accumulation in B and NK Lymphocytes in Relation to FCGR3B Copy Number

(A) FcγRIIb accumulation in B and NK cells of individuals with different FCGR3B copy number. The top plots show accumulation of FcγRIIb (2B6+) in B cells (CD19+) of FCGR3B two-copy (not carrying an FCGR3B-deleted haplotype), one-copy (carrying one deleted haplotype) and zero-copy (carrying two deleted haplotypes) individuals, respectively. The two 2B6+ populations (2B6low, 2B6bright) are indicated. The bottom plots show accumulation of FcγRIIb (2B6+) in NK cells (CD56+) of FCGR3B two-, one-, and zero-copy individuals. The two populations of CD56+ (CD56low, CD56bright) and 2B6+ (2B6low, 2B6bright) are indicated. The seemingly more pronounced CD56+ 2B6+ signal in one-copy individuals compared to zero-copy individuals is a result of the difference in the total cell count in the two samples. The quantitative flow cytometry data (Table S3) summarized in (B) shows that the percentages of NK cells on which FcγRIIb is detected (2B6+) are similar in one- and zero-copy individuals.

(B) Relative frequency of B and NK cells on which FcγRIIb is detected (2B6+) in individuals with different FCGR3B copy number. Shown is the mean percentage of CD19+ (B) and CD56+ (NK) cells on which FcγRIIb is detected (2B6+) in FCGR3B two-, one-, and zero-copy individuals, respectively. The number of individuals n analyzed is given below each plot. Whiskers indicate SD.