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. 2012 Dec 4;8:629. doi: 10.1038/msb.2012.59

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Copyright © 2012, EMBO and Macmillan Publishers Limited

This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.

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Examples of signaling proteins regulating glycans expression. (A) Lectin staining and glycan profiles of representative hit genes. Top: Glycan profiles of respective hit genes. Axis values indicate normalized lectin signals. Solid lines delineate the cutoff values (4 and −4). Middle: Lectin staining of cells transfected with control GFP and hit gene siRNAs. Bottom: Main glycan structures recognized by the respective indicated lectins. (B) Model of Golgi organization and glycosylation regulation. Colored shapes populating the Golgi illustrate the variety of glycosylation enzymes.