Figure 2.

Dnmt1 deletion alters progenitor distribution and cell cycle progression. (a–f) Immunolabeling of retinas show distribution of progenitor markers GFP (a and b) and proliferating cell nuclear antigen (PCNA, c and d) at P3, and M-phase marker PH3 (e and f) at P0. Arrow in f indicates an ectopic PH3⁺ cell. Scale bar, (a) for (a–f) 50 μm. gcl, ganglion cell layer; ipl, inner plexiform layer; vz, ventricular zone. (g–j) Flow cytometric analyses of cell markers and cell cycle properties at P2. (g) Representative flow cytometry profiles for GFP and cyclin-dependent protein kinase inhibitor p27^Kip1. (h) Fluorescence-activated cell sorting quantification of GFP⁺ cells and p27^Kip1+ cells among total cells. (i) Cell cycle distributions of GFP⁺ progenitors. (j) Quantification of p27^Kip1+ GFP⁺ cells among GFP⁺ cells in different cell cycle phases. Numbers of individual retinas analyzed in (h–j) are indicated in h. *P<0.05, **P<0.01, ***P<0.001