Figure 1.

Spatial and temporal expression profile of the endogenous mouse and human Umodl1 genes. (a) Schematic comparison of functional domains between mouse and human Umodl1 proteins. (b) Differential expression of human UMODL11 in normal and cancer tissues examined by Serial Analysis of Gene Expression (SAGE; adapted from http://www.genecards.org/cgi-bin/carddisp.pl?gene=Umodl; The SAGE analysis is accomplished by a joint effort by the Weizmann Institute of Science, the Salk Institute for Biological Studies and Tufts University ). (c–e) Umodl1 mRNA distribution detected by ISH. Paraffin sections of WT mouse tissues were tested with either ³⁵S- or digoxigenin-labeled riboprobes. Umodl1 signal was visualized by autoradiography (c and d) or alkaline phosphatase staining (e), respectively. c is the bright field view of the section in d. (f–k) Immunofluorescence showing Umodl1 in thymus. f and g are the same section dual-labeled with TCRβ and Umodl1. h is the merged f and g. (i–k) Dual immunofluorescence analysis of CD11c (i) and Umodl1 (j) co-expression in the APCs. k is the merged image of i and j. (l) Western blot analysis showing stable translation of Umodl1 in the proliferating splenic CD4⁺T cells and TCRβ⁺ thymocytes once stimulated by anti-CD3/CD28 antibodies. Untreated splenic CD4⁺ T cells and TCRβ⁺ thymocytes were included as the negative controls. β-actin was used as the internal loading control. (m) Northern blot showing the time course of mouse Umodl1 expression in vivo after gonadotropin stimulation. Size of the scale bars is indicated in the corresponding section. The asterisks show the human cancerous tissues in which Umodl1 is drastically up-regulated.