Figure 4.

Nec-1/1-M-Trp and Nec-1i, but not Nec-1s, inhibit IDO activity. (a) IDO enzymatic assay using recombinant IDO shows IDO inhibitory activities of Nec-1 and Nec-1i, but not of Nec-1s, which is in accordance with the predictions of the docking experiment. (b) Prototype IDO inhibitor D-1-MT has no inhibitory activity on cellular necroptosis assay using L929 cells. Cell survival was measured by MTT colorimetric method. (c) Increasing the concentration of 1-MT up to 100 μM has no effect on necroptosis. Cell survival was measured by MTT colorimetric method