Figure 3.

P-1257 inhibits PI3K/Akt until day 6 post-transfection. (a) Total cell extracts from MCF7 cells, control or transfected with P-1257, were separated by SDS-PAGE and analyzed by immunoblotting with anti-total (lower panel) or P-Akt (upper panel) from day 3 to 6 post-transfection. (b and c) Total cell lysates from KPL-4 and JIMT-1 cells untreated or treated with LY294002 at time 0 and after 48 h were separated by SDS-PAGE and immunoblotted with anti-total (lower panel) or P-Akt antibodies (upper panel) at different times as indicated in the figure