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. 2012 Dec 13;3(12):e442. doi: 10.1038/cddis.2012.180

Figure 5.

Figure 5

PRAP1 knockdown sensitizes cells to caspase-dependent apoptosis induced by 5-FU. HCT116 cells were transfected with either control siRNA or PRAP1 siRNAs for 48 h and followed by the addition of 5-FU at the indicated doses for 24 h. (a) The percentage of dead cells, indicated by sub-G1 proportion was determined by flow cytometry detection in FL2 channel after staining the cells with propidium iodide. Knockdown of PRAP1 expression enhances the cell death induced by 5-FU as compared with control siRNA-treated cells (*P<0.05). (b) PRAP1 knockdown increases caspase 3 activity induced by 10 μM 5-FU as compared with control siRNA-treated cells (*P<0.05). (c) PRAP1 knockdown enhances caspase-dependent apoptosis induced by 5-FU. The pan caspase inhibitor Z-VAD-FMK or Caspase 3-specific inhibitor was added 1 h before 5-FU treatment in PRAP1 knockdown cells. Inhibition of caspases significantly reduced the percentage of sub-G1 cells in PRAP1 knockdown cells, indicating that these cells die by caspase-dependent apoptosis (*P<0.05)