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. 2012 Dec 13;3(12):e444. doi: 10.1038/cddis.2012.184

Figure 2.

Figure 2

AUL12 inhibits OCR and RC complex I. (a) On the right, representative traces of OCR measurements performed on monolayers of living SAOS-2 cells treated with AUL12. Subsequent additions of the ATP synthase inhibitor oligomycin, of the uncoupler FCCP, of the RC complex I inhibitor rotenone and of the RC complex III inhibitor antimycin A were carried out. AUL12 at the reported concentrations was pre-incubated 15 min before starting the experiments. Bar graphs on the right show the coupled mitochondria respiration, which was calculated by subtracting the uncoupled mitochondrial OCR from the basal OCR (after and before oligomycin addition, respectively) after having detracted the non-mitochondrial component of OCR (the rotenone/antimycin insensitive OCR fraction). The enzymatic activity of RC complexes II–III (b) or of RC complex I (c) was evaluated on mitochondria isolated from SAOS-2 cells in the presence or absence of different concentrations of AUL12 added 5 min before starting the experiment. In (d), the activity of RC complex I was analyzed on permeabilized SAOS-2 cells pre-treated or not for 90 min with 40 μM AUL12. All data of RC complex activity were normalized to the citrate synthase activity and are mean±S.D. values (n=6). Statistical significance was measured with a Student's t test and is indicated by asterisks (**P<0.005)