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. 2012 Dec 20;3(12):e447. doi: 10.1038/cddis.2012.185

Figure 2.

Figure 2

Caspases are crucially involved in paracetamol plus TRAIL mediated endothelial cell death. skHep-1 or EA.hy926 cells were treated with paracetamol (10 or 20 mM), TRAIL (30ng/ml; respectively 3 ng/ml) or both for 6 h, and the protein level of cleaved caspase 3 was analyzed by western blot (a). Tubulin was used to normalize protein loadings. Cell death was assessed by DEVD cleavage assay 7 h after stimulation (b). Cell death was assed by microscopy after Hoechst 33342 and PI staining (c), or by MTT assay (d). skHep-1 or EA.hy926 cells were pretreated (30 min) with 10 μM caspase inhibitor (ZVAD, ZV) and stimulated with increasing concentrations of paracetamol, 30, respectively 3 ng/ml TRAIL, or both for 16 h. Mean values±S.D. triplicates are shown for MTT and DEVDase assays. A typical experiment out of five is shown for cleaved caspases 3. Representative pictures are shown for microscopic analysis. ***P<0.001, **P<0.01 versus paracetamol or paracetamol and TRAIL stimulation