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Transient expression of ISG56/IFIT1 inhibits PIV5. Hep2 cells were transiently transfected with a plasmid containing haemagglutinin (HA)-tagged ISG56/IFIT1. At 24 h post-transfection, cells were either mock infected or infected with PIV5 at a high m.o.i. (10 p.f.u. per cell). At 24 h p.i., the cells were fixed and immunostained for the viral NP protein (red) and the HA-tagged ISG56/IFIT1 (green).
