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Analysis of the relative levels of CPI− mRNAs in Hep2/BVDV-Npro and Hep2/BVDV-Npro/shISG56/IFIT1-infected cells that were pre-treated (IFN pre) or not (−IFN) with IFN at 8 h prior to infection with CPI−, or were treated with IFN at 8 h p.i. (IFN post). At 20 h p.i., total cell RNA was extracted and the levels of NP, P/V, M, HN and L mRNA relative to β-actin mRNA were determined by qPCR. Results are shown as means±sem of duplicate samples in two separate experiments.
