Table 2. Analysis of media metal content.

Brucella broth (BB) and HAMS F-12 ICP-MS metal measurements derived from two technical replicates are shown. Complete BB10 and HAMS10 metal contents were calculated from Brucella broth or a 0.8× stock solution of HAMS F-12 plus 10 % FBS. −, Readings <1 µM.

Metal	FBS (μM)	BB (μM)	BB10 calculated (μM)	HAMS (μM)	HAMS10 calculated (μM)
Cr-52	0.011	0.913	0.823	0.001	0.002
Fe-56	16.397	8.954	9.698	2.387	3.788
Co-56	0.001	0.059	0.053	0.379	0.341
Ni-60	−	−	−	−	−
Cu-63	1.084	0.151	0.244	−	0.043
Zn-66	15.855	5.173	6.240	2.747	4.058