Table 3. Biomass, sterigmatocystin, RS and antioxidant enzyme production in A. nidulans ΔnapA and OE : : napA mutants.

WT, wild-type.

Strain and treatment	Physiological parameter*
	Biomass (DCM†) production (g l−1)			Sterigmatocystin production [mg (g DCM)−1]	RS‡ production [pmol DCF (g DCM)−1]		Specific antioxidant enzyme activities
							Catalase [kat (kg protein−1)]		Glutathione peroxidase (GPx) [mkat (kg protein)−1]
	0 h	10 h	24 h	24 h	10 h	24 h	10 h	24 h	10 h	24 h
WT	2.0±0.3	4.5±0.6	4.2±0.5	1.5±0.5	0.25±0.05	0.19±0.05	0.21±0.02	0.28±0.03§	0.54±0.05	0.31±0.03§
WT+tBOOH	2.1±0.3	2.2±0.6¶	4.4±0.5§	3.1±1¶	27±5¶	0.52±0.06§¶	0.56±0.05¶	0.31±0.04§	0.68±0.05¶	0.28±0.03§
ΔnapA	2.1±0.3	4.4±0.3	3.6±0.4§	1.4±0.5	0.41±0.06#	0.47±0.07#	0.12±0.02#	0.20±0.03§#	0.68±0.07#	0.33±0.03§
ΔnapA+tBOOH	2.1±0.2	1.8±0.3¶	3.4±0.4§#	3.3±1¶	32±7¶	1.8±0.5§¶#	0.31±0.03¶#	0.28±0.03¶	0.76±0.08	0.65±0.07¶#
Pyridoxine supplementation
WT	2.2±0.3	4.5±0.6	4.2±0.5	1.6±0.5	0.24±0.05	0.17±0.05	0.22±0.02	0.27±0.04	0.51±0.05	0.21±0.03§||
WT+tBOOH	2.1±0.2	4.6±0.6||	4.0±0.5	3.0±1¶	19±4||¶	0.44±0.05§¶	0.39±0.02||¶	0.28±0.03§	0.63±0.05¶	0.23±0.03§
OE : : napA	2.3±0.3	4.8±0.5	4.0±0.5	0.7±0.3**	0.3±0.05	0.19±0.04§	0.19±0.02	0.26±0.03§	0.62±0.06**	0.53±0.07**
OE : : napA+tBOOH	2.2±0.3	4.8±0.5	4.2±0.5	1.0±0.3¶**	1.1±0.2¶**	0.18±0.05§**	0.21±0.02**	0.25±0.03	0.68±0.06	0.47±0.07§**

^*Mean±sd values calculated from three independent experiments are presented.

^†DCM, dry cell mass.

^‡RS includes all intracellular oxidants which react to give rise to 2′,7′-dichlorofluorescein from from 2′,7′-dichlorofluorescin diacetate (Halliwell & Gutteridge, 2007).

^§||¶#**Significant differences (P<0.05, where P values were calculated using Student’s t test) between 10 and 24 h incubations (§), caused by pyridoxine (||) supplementation or tBOOH treatment (¶) or initiated by ΔnapA (#) or OE : : napA (**), respectively.