Fig. 5.

Mapping of the 3′ end of positive-stranded viral RNAs. The 3′ ends of wt and recombinant BUNV RNAs were determined by nucleotide sequencing of TA-cloned 3′-RACE products. Depicted at the top is a schematic representation of the 3′ UTR of the BUNV S antigenomic RNA (nt 840–961). Nucleotide sequences of the regions with termination hot spots (840–868 and 952–961) are in boxes. For each clone the sequence is aligned to the schematic on top, followed by either the number of the 3′ nucleotide or the region at which termination could have occurred (and which is underlined in the sequence). The number in parentheses indicates how often an identical sequence was identified.