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. 2013 Jan 7;200(1):109–123. doi: 10.1083/jcb.201207012

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Figure 7. — The transition from lipid mixing to syncytium formation depends on cell metabolism. (A and B) A mix of NaN₃ and 2-d-deoxyglucose was applied to fusion-committed C2C12 cells 5 min before LPC removal. (A) Images of the cells treated (bottom) and untreated (top) with ATP-depleting mix were taken 30 min after LPC removal. Left, phase contrast with nuclear staining; right, DiI (red) and green cell tracker. Red and white arrows mark the multinucleated cells and colabeled mononucleated cells, respectively. Bar, 50 µm. (B and C) In contrast to lipid mixing (green), syncytium formation (red) is blocked by ATP depletion for both C2C12 cells (B) and primary myoblasts (C). Fusion extents for the ATP-depleted cells (2) were normalized to those for the untreated cells released from LPC block (1). All results are shown as means ± SEM (n ≥ 3). Levels of significance relative to controls (1) are shown: **, P < 0.01.