Table 2. Comparison of Km values and specific activity of EtCRK2 in the two protein–activator complexes.
Parameter | EtCRK2/XlRINGO | EtCRK2/EtCYC3a |
Specific activity (µmol min−1 mg−1) | 1.29±0.24 | 0.15±0.02 |
Km for ATP (µM) | 9.39±1.21 | 9.05±0.76 |
Km for fluorescein-labelled substrate (µM) | 1.72±1.10 | 2.05±1.18 |