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. 2012 Sep;158(Pt 9):2303–2314. doi: 10.1099/mic.0.059931-0

Fig. 2.

Fig. 2.

Deletion of exsE results in loss of HeLa cell cytotoxicity. The indicated strains were used to infect HeLa cells cultured in DMEM supplemented with 1 % FBS (v/v) at an m.o.i. of 100 for 4 h and assayed for T3SS1-dependent cytotoxicity. Percentage cytotoxicity was calculated based on the release of LDH relative to the uninfected control (0 %) and wild-type NY-4 strain (100 %). The reported values represent the mean±sem for three independent replicates. Asterisks, statistically significant difference in mean value compared with wild-type NY-4 by one-way ANOVA (P<0.05).