Fig. 3.

Deletion of exsE results in loss of LC3 conversion. The indicated strains were used to infect HeLa cell monolayers at an m.o.i. of 100. Whole-cell lysates were separated using 4–20 % SDS-PAGE, transferred to a membrane and probed using anti-LC3 and anti-actin antibody. Densitometry was used to determine relative LC3-II accumulation and to calculate the LC3-II/I ratio. The reported values represent the mean±sem for three independent experiments. Asterisks, statistically significant difference in mean value compared with wild-type NY-4 by one-way ANOVA (P<0.05).