Fig. 7.

Deletion of exsE does not affect relative transcription levels of exsA or lafA. HeLa cell monolayers were infected with either NY-4 or ΔexsE at an m.o.i. of 100. Total RNA was extracted and cDNA was synthesized for qPCR analysis as described in the main text. Reported values represent the mean±sem for relative expression level of four biological replicates at each time point. Asterisks, statistically significant difference in mean value compared with t = 0 h by one-way ANOVA.