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. 2012 Sep;158(Pt 9):2303–2314. doi: 10.1099/mic.0.059931-0

Table 2. Primers used in this study.

Primer Sequence (5′–3′)
vp1702-1F AGGTTACTCGAGTCCCCACGGACTGAGCGCAT
vp1702-1Rre AGGATATCTAGAAAGACACCTAAACTC
vp1702-1Fre AGGATATCTAGAACACAAGTTATCACGCAC
vp1702-2R GAGTTTAGGTGTCTTACACAAGTTATCACGCAC
delta-exsEseq up TGGCCGAAGAGACGCCTGCT
delta-exsEseq down GGGGATCGGGCGTGAAACGC
lfgE-1F AGGATAAACTCGAGTTCGCTACCGTGTATCAAAAAAC
lfgE-1R CTATCCATGCAGAACTCCTAAAAAGACCTCTGATTTGCTT
lfgE-2F AAGCAAATCAGAGGTCTTTTTAGGAGTTCTGCATGGATAG
lfgE-2R AGTTAGTCTAGACGTGCCGACAACGCGCACAC
lfgE-F CTTGGCTTAAAGCCGGGCAA
lfgE-R CGCGTTGATGCAGAGTTTGTGAC
P1668F-XbaI AGGATATCTAGAAATACTCATTCACTTGCACTC
P1668R-BamHI AGTTAGGGATCCAATGTAAAAAATATGCGCAATG
P1668R-BglII AGTTAGAGATCTAATGTAAAAAATATGCGCAATG
vp1702F-BamHI AGGATAGGATCCATGTCTAATGACATCCAATC
vp1702R-BglII AGTTAGAGATCTTTAATGGTGATGGTGATGGTGCCTTTCGCTTCGAGCAA
1656-up AGGATAGAATTCAGGAGATATACCATGTTGGATAAAATTGGTGGAAC
1656-down AGTTAGTCTAGATTAATGGTGATGGTGATGGTGCACTGTCGGGATAGATGCGC
1680–600-up TCTACGTTCCATAAAGGCCAC
1680–600-down CAACATCGCGTGACCAATGG
1686–600-up ATTCTAAATGAAGGCAAACTCAGC
1686–600-down GTTTAAATCCGTACTTGCGAGC
SecYF TGGTGCTCTTGAGCGTGCATC
SecYR CCTTGTTGACGCTTCGCGTAG
qExsA-F TCCGTCAGCTTCCACTCTTT
qExsA-R CTCGGGCTTGTTTTCTTTTG
qExsE-F AACGTTTCAAGGTCGCAAAG
qExsE-R TACCTTTCGCTTCGAGCAAT
qLafA-F CGCAGCTATCACTGACGGTA
qLafA-R TCCATGATACGGCCTTTAGC
qSecY-F ACTGGCTCAGTGGTTTGGTC
qSecY-R GGGTACGAATGCACCAGACT