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. 2012 Sep;158(Pt 9):2353–2362. doi: 10.1099/mic.0.058206-0

Fig. 1.

Fig. 1.

Amplification and sequence analysis of the mosaic tet(S/M) gene. (a) PCR amplification with primers directed outwards from tet(S/M). Lanes: 1, no DNA control; 2, size marker (1 kb ladder); 3–5, template DNA from S. bovis independent isolates (1315, 1357 and 1400; Devirgiliis et al., 2010). (b) Sequence alignment of mosaic tet(S/M) with tet(S) (accession no. X92946) and tet(M) (U09422), including the portion of coding sequence where fusion occurred between the two tet genes and the untranslated regulatory region upstream of the tet(S) ATG. Transcriptional and translational regulatory sequences (annotated in X92946) are boxed. Mosaic tet(S/M) and tet(M) ATG start codons are underlined. Amino acid residues contributed by Tet(S) in the mosaic protein are shown in bold type.