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. 2013 Jan 9;449(Pt 3):673–681. doi: 10.1042/BJ20120952

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© 2013 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) WT (HT1) and pph3Δ (HT2) G₁ cells were released into conditions for yeast (YF) or hyphal (HF) growth. Aliquots of cells were harvested at the indicated times for Western blot analysis of Rfa2. (B) WT (HT1), pph3Δ (HT2) and psy2Δ (HT3) G₁ cells were first treated with 20 mM HU and then allowed to recover by transferring cells to HU-free medium for further growth. Aliquots of cells were harvested at the times indicated during both HU treatment and the recovery period for Western blot analysis of Rfa2.