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. 2013 Jan 9;449(Pt 3):673–681. doi: 10.1042/BJ20120952

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© 2013 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Top panel: Rfa2 affinity-purified from WT (HT41), pph3Δ (HT42) and psy2Δ (HT43) cells was incubated with FITC-labelled dsDNA at molar ratios of DNA/protein of 0, 8, 16 and 32 at room temperature for 30 min. The protein/DNA mixture was then resolved by TGE/PAGE (8% gel) and detected by fluorescence at an emission wavelength of 320 nm. Bottom panel: the same amounts of purified Rfa2 as above were resolved by SDS/PAGE (12% gel) as a protein loading control. (B) The experiment described above was repeated using ssDNA instead of dsDNA.