Table 3. Comparison of allele frequencies for contingency genes Cj0170, Cj0045 and Cj1420 in single-colony isolates prepared from glycerol stocks or prepared for mouse inocula.

Isolate	Cj0170		Cj0045		Cj1420
	Glycerol*	Inoculum†	Glycerol	Inoculum	Glycerol	Inoculum
SCK1‡ (S)	81 (10G)	78
SCK7 (S)	84 (10G)	80
SCK18 (U)	90 (9G)	89
SCK21 (U)	90 (9G)	90
SC4‡ (S)			86 (9G)	85	97 (9G)	100
SC13 (S)			96 (9G)	84	100 (9G)	95
SC5 (U)			72 (11G)	58	96 (8G)	92
SC9 (U)			71 (11G)	53	97 (8G)	95

^*Percentage frequency of the predominant allele in population derived from glycerol stock (see Methods).

^†Percentage frequency of the predominant allele in population after culture in growth medium during preparation of inocula for oral gavage into mice.

^‡Single-colony isolates SCK1 and SCK7 were derived from a successful population of C. jejuni KanR4 and carry the successful allele (S) of Cj0170 (10G HT). Single-colony isolates SCK18 and 21 were derived from the same successful KanR4 population and carry the unsuccessful allele (U) of Cj0170 (9G HT). Single-colony isolates SC4 and SC13 were derived from a successful population of C. jejuni 33292 and carry successful alleles (S) of Cj0045 (9G HT) and Cj1420 (9G HT). Single-colony isolates SC5 and SC9 were derived from the same successful population of C. jejuni 33292 and carry unsuccessful alleles (U) of Cj0045 (11G HT) and Cj1420 (8G HT).