Table 4. Shifts in allele frequency in contingency gene Cj0045 in single-colony isolates of 33292 during mouse passage.
Experiment | Single-colony isolates | Non-passaged or mouse passaged | Number of mice | Allele frequency (%) in Cj0045 HT* | |||||
<8G | 9G | 10G | 11G | 12G | >13G | ||||
G1 | 33292-SC5 | Non-passaged | na† | 3 | 28 | 66‡ | 3 | ||
Mouse passaged | 2 | 10 | 4 | 24 | 61 | 2 | |||
Mouse passaged | 1 | 7 | 6 | 85 | 2 | ||||
33292-SC13 | Non-passaged | na | 15 | 85 | |||||
Mouse passaged | 3 | 12 | 88 | ||||||
G2 | 33292-SC5 | Non-passaged | na | 2 | 3 | 34 | 58 | 3 | |
Mouse passaged | 5 | 7 | 83 | 8 | 1 | 1 | |||
Mouse passaged | 1 | 9 | 41 | 49 | 1 | ||||
Mouse passaged | 1 | 4 | 30 | 63 | 3 | ||||
33292-SC9 | Non-passaged | na | 2 | 5 | 37 | 53 | 3 | ||
Mouse passaged | 4 | 10 | 79 | 9 | 1 | 2 | |||
Mouse passaged | 1 | 1 | 5 | 26 | 65 | 3 | |||
33292-SC4 | Non-passaged | na | 8 | 85 | 7 | ||||
Mouse passaged | 5 | 9 | 87 | 4 | |||||
Mouse passaged | 1 | 5 | 15 | 77 | 3 | ||||
33292-SC13 | Non-passaged | na | 13 | 84 | 3 | ||||
Mouse passaged | 7 | 9 | 88 | 2 |
Fragment analysis was performed on C. jejuni in the mouse inoculum (non-passaged) or recovered from individual mice (mouse-passaged). Numbers represent the mean allele frequencies.
na, Not applicable.
Predominant alleles in a population are in bold type. SC5 and SC9 mouse inocula carry the 11G unsuccessful allele as the predominant allele. SC4 and SC13 mouse inocula carry the 9G successful allele as the predominant allele.