Fig. 1.
BsYjbH mutants show similar activity to wild-type BsYjbH. (a) Effect of the wild-type and mutant on Spx-dependent expression of trxB–lacZ. The graph shows the IPTG induction time-course of β-galactosidase activity encoded by the trxB–lacZ fusion in yjbH mutant cells bearing the Physpank–BsyjbH (white) and mutants [coloured (see key)] or the Physpank promoter (black) in a yjbH-null mutant background. β-Galactosidase activity is expressed as Miller units. (b) Immunoblot analysis of Spx level using anti-Spx antiserum (Nakano et al., 2002). Cell samples were collected before IPTG induction and at 1 h after IPTG induction from the strains in (a).