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. 2012 May;158(Pt 5):1379–1387. doi: 10.1099/mic.0.057869-0

Fig. 2.

Fig. 2.

Construction of M. marinum ΔpapA5. (a) Scheme of papA5 deletion cassette (ΔpapA5c)-delivery suicide vector used for construction of M. marinum ΔpapA5. The papA5 deletion leaves behind a gene remnant consisting of the first two (black type) and last two (white type) amino acids of PapA5. The gene remnant in ΔpapA5c is flanked by WT sequence for homologous recombination with the chromosome. (b) Agarose gel electrophoresis showing PCR-based confirmation of the papA5 deletion in M. marinum ΔpapA5. Lanes: 1, M. marinum WT (2270 bp amplicon expected with primers papA5OF and papA5OR); 2, M. marinum ΔpapA5 (1035 bp amplicon expected with papA5OF and papA5OR); 3, M. marinum WT (1275 bp amplicon expected with primers papA5F and papA5R); 4, M. marinum ΔpapA5 (no amplicon expected with papA5F and papA5R); L, DNA ladder marker.