FIG. 3.
Short stimulation with BMP-2 did not affect the attachment of human SVF cells to calcium phosphate scaffolds. (A) A 15-min BMP-2 pretreatment did not change the cell number (expressed as DNA, ng) of hASCs on different BCP and β-TCP scaffolds after allowing attachment for 30 min. More hASCs seemed to attach to the BCP scaffolds compared to β-TCP scaffolds. (B) A 15-min BMP-2 pretreatment affected the percentage of CFU-f from nonattached cells dependent on the scaffold. More BMP-2-treated cells were attached to BCP 20/80 (p=0.05) and β-TCP>0.7 mm (p=2.2×10−7). Values are calculated relative to the CFU-f proliferation assay data (Fig. 1). Values are mean±SEM (n=6). Significant difference between scaffolds, *p<0.05, ***p<0.001. SVF, stromal vascular fraction; BCP, biphasic calcium phosphate; β-TCP, β-tricalcium phosphate.