FIG. 2.

Tissue-free control scaffolds seeded with culture expanded meniscus cells. (A) Scanning electron microscopy images of scaffold fiber morphology (scale=20 μm). Cross section of scaffolds with (B) DAPI staining for cell nuclei and (C) Picrosirius red/Alcian blue staining for collagen/proteoglycan deposition (scale=200 μm). (D) Quantification of DNA (top) and glycosaminoglycan (GAG) (bottom) content. Bars above indicate significant difference between groups (where the lack of vertical bar indicates origin for comparison to other groups), with p<0.05; n=6–7/condition. Color images available online at www.liebertpub.com/tea