FIG. 4.

(a) The calcium content of ADSCs on PCL/PLGA and PCL/PLGA/β-TCP membranes on day 21, determined using a calcium assay kit. Alizarin Red S staining of (b, c) PCL/PLGA and (d, e) PCL/PLGA/β-TCP membranes with ADSCs at day 21, and (f) PCL/PLGA/β-TCP membranes without ADSCs. (g) The quantitative analysis of Alizarin Red S staining. The calcium content and staining intensity of ADSCs were significantly higher on the PCL/PLGA/β-TCP membranes than on the PCL/PLGA membranes. Alizarin Red S did not stain the β-TCP mixed with PCL/PLGA. There were three replicates in each group for the statistical analysis. Color images available online at www.liebertpub.com/tea