FIG. 2.

(a) Hematoxylin and eosin (H&E)-stained section of PDLs seeded in a nonwoven 50:50 blend of polyglycolic acid–poly-l-lactic acid scaffold showing the distribution of cells within the three-dimensional culture environment. (b) qRT-PCR-derived expression (average±standard error of the mean [SEM]) of PDLs for assessment of embryonic stem cell markers. Group 1: Cells cultured in regular basal medium. Group 2: Cells cultured in differentiating medium. Group 3: Cell cultured in basal medium and exposed to 1 dyne/cm². Group 4: Cells cultured in differentiating medium and exposed to 1 dyne/cm². Each column is normalized by GAPDH. (c) Same analysis as part (b) repeated for smooth muscle cell and endothelial cell markers. (d) Same analysis as part (b) repeated for osteogenic, chondrogenic, and collagen type 1 and 3 markers. The initial sample size was an n=4 wells of standard-sized 8-well plate/group. However, to enable sufficient detection limits for qRT-PCR analysis, specimens were pooled into an n=2–3 samples/group with an r=2–3 measurements/sample. qRT-PCR, quantitative real-time-polymerase chain reaction. Color images available online at www.liebertpub.com/tea