Table 1.
Quantitative RT-Polymerase Chain Reaction Primer Sequences Utilized in This Study
| Gene | Forward primers (5'-3') | Reverse primers (5'-3') |
|---|---|---|
| GAPDH | AATGAAGGGGTCATTGATGG | AAGGTGAAGGTCGGAGTCAA |
| Oct4 | CTCCTGAAGCAGAAGAGGATCAC | CTTCTGGCGCCGGTTACAGAACCA |
| SOX2 | TGCAGTACAACTCCATGACCA | GTGCTGGGACATGTGAAGTCT |
| NANOG | GTCTTCTGCTGAGATGC | AGTTGTTTTTCTGCCACC |
| KLF4 | ACGATCGTGGCCCCGGAAAAGGAC | TGATTGTAGTGCTTTCTGGTGGGCTCC |
| nestin | GCCCTGACCACTCCAGTTTA | GGAGTCCTGGATTTCCTTCC |
| SLUG | TGCTACACAGCAGCCAGATTCC | TTTCTGGGCTGGCCAAACAT |
| p75 | CTGCAAGCAGAACAAGCAAG | GGCCTCATGGGTAAAGGAGT |
| SOX10 | TCTTGTAGTGGGCCTGGATGG | TGAACGCCTTCATGGTGTGG |
| Integrin-β1 | CCTACTTCTGCACGATGTGATG | CCTTTGCTACGGTTGGTTACATT |
| THY1 | TCGCTCTCCTGCTAACAGTCT | CTCGTACTGGATGGGTGAACT |
| PECAM1 | CCAAGGTGGGATCGTGAGG | TCGGAAGGATAAAACGCGGTC |
| FLK1 | GGCCCAATAATCAGAGTGGCA | CCAGTGTCATTTCCGATCACTTT |
| VE-cadherin | GATCAAGTCAAGCGTGAGTCG | AGCCTCTCAATGGCGAACAC |
| TIE-1 | TCGAGCGGCATCTACAGTG | GCACGATGAGCCGAAAGAAG |
| FZD2 | CGGCCCCGCAGCGCCCTGCCC | ACACGAACCCAGGAGGACGCAGGCC |
| MLC1F | GAGTTCTCTAAGGAACAGCAGG | CTGCGTGTCTTTGACAAGGAAGGCAATGG |
| ALP | GTTGCCAAGCTGGGAAGAACAC | CCCACCCCGCTATTCCAAAAC |
| osteocalcin | CACTCCTCGCCCTATTGGC | CCCTCCTGCTTGGACACACAAAG |
| COL1A1 | TACCATGACCGAGACGTGTG | ATAAGACAGCTGGGGAGCAA |
| COL2A1 | AGACTTGCGTCTACCCCAATC | GCAGGCGTAGGAAGGTCATC |
| COL3A1 | GACATCGAGGATTCCCTGGT | CCAATCCCAGCAATGGCAG |
All sequences were obtained using the BLAST program, National Center for Biotechnology Information (NCBI), except for the markers FZD2 and MLC1F, which were obtained from Brand et al.22
RT, real time.