Table 1.
Surface epitopes for RS cells and mMSCs
| Epitopes | RS-1 cells | RS-2 cells | mMSCs |
|---|---|---|---|
| Selective for RS cells | |||
| Vascular endothelial growth factor receptor (FLK-1) | (+) | (+/−) | (−) |
| TRK (C-14; a nerve growth factor receptor) | (+) | (+/−) | (−) |
| Transferrin receptor | (+) | NA | (−) |
| Annexin II (Lipocortin 2) | (+) | NA | (−) |
| Multidrug resistance | (+/−) | (+/−) | (−) |
| Epithelial membrane antigen | (+/−) | (+/−) | (−) |
| CD4 | (+/−) | (+/−) | (−) |
| CD104 | (+/−) | (+/−) | (−) |
| CD117 (c-Kit; stem cell factor receptor) | (+/−) | (+/−) | (−) |
| Selective for mMSCs | |||
| STRO-1 | (−) | (−) | (+/−) |
| PDGF-R | (−) | (−) | (+/−) |
| EGF-R | (−) | (−) | (+/−) |
| CD10 | (−) | (−) | (+) |
| CD147 (Neuroregulin) | (−) | (−) | (+) |
| Nonselective | |||
| Annexin V (Lipocortin 5) | (+/−) | NA | (+/−) |
| HLA-1 | (+/−) | (+/−) | (+/−) |
| Basic FGF receptor | (+/−) | (+/−) | (+/−) |
| CD31 | (+/−) | (+/−) | (+/−) |
| CD38 | (+/−) | (+/−) | (+/−) |
| CD44 (Hyaluronic acid receptor) | (+) | (−) | (+) |
| CD49e (integrin alpha 5) | (+) | (+) | (+) |
| CD59 | (+) | (−) | (+) |
| CD81 | (+/−) | (−) | (+) |
| CD90 (Thy-1) | (+/−) | (−) | (+) |
(+), most cells positive; (+/−), some cells positive; (−), negative. All three subpopulations of MSCs are negative for the hematopoietic markers: CD1a, CD11B (Mac-1), CD14, CD27, CD34, CD43, CD45, and CD133. They were also negative for a series of other markers: CD50 (I-CAM 3), CD53, CD109, CD114 (G-CSFR), HLA-2, CCR5 (chemokine receptor-5), and human L1 (neurite adhesion molecule).