FIG. 4.

Effect of coculture of HBDC with HUVEC grown in direct contact on ALP activity. (A) Data obtained in one from the five experiments are shown as the mean values (n=6). Error bars represents the 95% CI of a mean.***p<0,001, ns, not statistically significant. (B) Values obtained in all cocultures are normalized to the ALP activity in HBDC monoculture. Mean values from five independent experiments are shown; n=30 (five experiments×six repetitions). For each repetition cells from different donors were used. CIs and standard errors for relative ALP activity have been shown. The CIs do not include 1, so ALP activity is significantly higher in cocultures than in HBDC culture. Initial percentage HUVEC:HBDC is shown at the x-axis. ALP, alkaline phosphatase.