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. 2012 Oct 4;19(3-4):393–402. doi: 10.1089/ten.tea.2011.0710

FIG. 7.

FIG. 7.

VEGF release (A) and VEGF mRNA expression (B) in the investigated cultures. (A) Data are shown as the mean from three independent experiments. Error bars represents the 95% CI of a mean (n=9). The amount of VEGF released in HBDC monoculture compared with other type of culture was significantly higher (p<0,001, while there were no statistically significant differences between HUVEC monoculture and 1:1 or 1:4 coculture. Significance of the other differences are marked: *p<0.05 and ***p<0.001). Two-way ANOVA and post-hoc Tukey test has been performed. Initial percentage HUVEC:HBDC is shown at the x-axis. (B) VEGF mRNA expression was normalized to GAPDH. HBDC monoculture on day 1 was used as a relative control. Data are shown as the mean (n=18).Error bars represent the 95% CI of a mean. The results show data from three independent experiments (n=18). The Kruskal–Wallis test was used for statistical analysis of data. Initial percentage HUVEC:HBDC is indicated in the legend. VEGF, vascular endothelial growth factor.