Analysis of toluene vapor concentration by gas chromatography. A, Schematic of experimental design. Toluene exposures are marked by the gray boxes. B, Sample chromatogram from 5000 PPM. H, Hexane; T, toluene; X, o-xylene. C, Standard curve showing the ratio of toluene and o-xylene area under the curve (r2 = 0.991; n = 5 for each). Open circles represent the measured concentrations from 4 and 8% toluene exposures in the vapor chamber (n = 5 for each). D, Representative chromatogram from an air sample from the vapor chamber when 8% toluene is flowing at a rate of 4 L/min for 10 min.