Movie 2.

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DOI: 10.1523/JNEUROSCI.3896-12.2013.video.2

Infrequent Ca²⁺ transients in the vulval muscles of wild-type animals before the egg-laying active phase. Intensity-modulated ratiometric imaging of wild-type C. elegans expressing the Ca²⁺ sensor GCaMP3 and soluble mCherry in the vulval muscles from the unc-103e promoter at 30 fps. Change in the GCaMP3 to mCherry fluorescence ratio is indicated by a rainbow scale from 0 (dark blue) to 1.2 (red). A twitch Ca²⁺ transient in the anterior vulval muscle cells was observed at ∼20 s. Still images from this video are shown in Figure 7A–C, and the traces of ΔR/R and vulval muscle area are shown in Figure 7F (left).