Movie 3.

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DOI: 10.1523/JNEUROSCI.3896-12.2013.video.3

Frequent, rhythmic Ca²⁺ transients in the vulval muscles of wild-type animals during the egg-laying active phase. This is the same animal as shown in Movie 2 and Figure 7A–F, recorded approximately 4 min later. Change in the GCaMP3 to mCherry fluorescence ratio is indicated by a rainbow scale from 0 (dark blue) to 1.2 (red). Twitch and egg-laying Ca²⁺ transients are indicated. Still images from this video are shown in Figure 7D,E, and the traces of ΔR/R and vulval muscle area are shown in Figure 7F (right). This video includes one egg-laying event at ∼15 s.