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. 2012 Dec 3;288(2):1047–1054. doi: 10.1074/jbc.M112.397521

FIGURE 3.

FIGURE 3.

Determination of proton translocation rate of PL vesicles embedded with wild-type or mutant bc1 complexes using a stopped-flow spectrophotometer. Reaction conditions were as described under “Experimental Procedures.” pH changes were monitored by following the absorption change of pyranine at 457 nm.